Leading London and Surrey Carboxytherapy services and training Academy: The Bohr Effect in Carboxytherapy: Oxygen, Regeneration, and Clinical Excellence

Published by Levitas Academy — Training Tomorrow’s Regenerative Clinicians 

 Clinical applications available at Levitas Clinics London, Esher, and Guildford 

Carboxytherapy — the therapeutic use of medical-grade carbon dioxide (CO₂) — has become a cornerstone in regenerative aesthetics. Its effects on microcirculation, collagen stimulation, and tissue oxygenation are rooted in one of physiology’s most elegant principles: the Bohr Effect. 

At Levitas Academy, our clinician-led training explores this science in depth, ensuring every practitioner understands the biochemistry behind the treatment. Levitas Clinics, our clinical arm, deliver carboxytherapy within tailored regenerative programs, aligning evidence with real-world outcomes. 

The Science: Understanding the Bohr Effect 

In 1904, Danish physiologist Christian Bohr described how increased CO₂ levels or decreased pH cause hemoglobin to release oxygen more readily — the Bohr Effect.  In simple terms, CO₂ acts as a biological key, unlocking oxygen from hemoglobin and allowing it to diffuse efficiently into tissues. 

During carboxytherapy, localised administration of CO₂ increases capillary CO₂ concentration, lowering pH and triggering this rightward shift of the oxygen–hemoglobin dissociation curve.  The result: a surge in tissue oxygenation, improved metabolism, and enhanced healing capacity. 

Mechanisms of Action in Skin and Tissue Regeneration 

1. Microvascular Expansion  CO₂ induces vasodilation, improving blood flow and capillary recruitment in treated areas. 

2. Improved Oxygen Delivery  Through the Bohr Effect, oxygen release from hemoglobin increases dramatically, correcting tissue hypoxia. 

3. Enhanced Collagen and Elastin Synthesis  Fibroblast activity is stimulated via mild, controlled hypoxia — initiating collagen remodeling and skin tightening. 

4. Increased Cellular Metabolism  Elevated O₂ and improved perfusion accelerate wound healing, scar reduction, and cellular regeneration. 

5. Lymphatic Drainage and Detoxification  Carboxytherapy supports the removal of inflammatory by-products and aids lymphatic flow, complementing detox and repair protocols at Levitas Clinics. 

Clinical Applications 

Modern evidence supports carboxytherapy for: 

• Periorbital rejuvenation (dark circles, under-eye puffiness) 

• Cellulite and stretch marks 

• Skin laxity and fine lines 

• Scarring and post-surgical recovery 

• Adjunctive therapy in fat grafting and regenerative dermatology 

Combination therapies — for example, PRP + Carboxytherapy or Exosomes + CO₂ Therapy — are now at the forefront of integrated aesthetic practice at Levitas Clinics, magnifying tissue response and repair. 

Safety and Tolerability 

Carboxytherapy is minimally invasive and safe when performed by trained clinicians.  Typical effects (transient erythema, warmth, mild discomfort) resolve rapidly. Contraindications include local infection, pregnancy, or uncontrolled vascular disease.  Levitas training ensures practitioners understand anatomical landmarks, depth, and pressure calibration, ensuring both safety and clinical precision. 

Training at Levitas Academy 

Accreditation and CPD 

• All Levitas Academy courses, including Carboxytherapy & The Bohr Effect, are CPD-certified. 

Faculty Credentials 

Training is delivered by clinicians actively practicing in regenerative medicine, including: 

• Dr. Ash Kapoor – Longevity & Regenerative Physician, Founder of Levitas One 

• Dr. Simone Kuter, Dr. Elizabeth Almas, Dr. Vijay Prakash, and Dr. Federico Fedel – experienced longevity and aesthetics practitioners affiliated with Levitas Clinics London & Surrey 

Certification 

Participants receive a Certificate of Completion, verifying CPD hours and suitable for professional appraisal and revalidation. 

Where Science Meets Practice 

Through Levitas Academy, medical professionals gain mastery in the mechanisms, safety, and clinical integration of carboxytherapy.  Through Levitas Clinics, patients benefit from the same evidence-based protocols, tailored to aesthetic restoration and regenerative wellness. 

Covering  

London : Knightsbridge , Kensington, Chelsea, Park Lane, Belgravia, Mayfair,Marylebone,,Westminster  

Surrey : Wimbledon, Epsom, Ashtead, Guildford, Hazelmere , Farnham , Godalming   Together, these institutions form the educational and clinical pillars of modern regenerative medicine. 

Key References 

1. Jarząbek-Perz S, Wrzosek M. Carboxytherapy in the Management of Selected Skin Conditions – Applications in Monotherapy and Combined Treatments. Appl Sci. 2025;15(17):9236. 

2. Bagherani N, et al. An Overview of the Role of Carboxytherapy in Dermatology. J Cosmet Dermatol. 2024;23(5):e15741. 

3. Borile G, et al. Carboxytherapy in Aesthetic and Regenerative Dermatology. In: Advances in Aesthetic Medicine. Springer; 2024. 

4. Limongi RM, et al. Carboxytherapy in Dermatology: Mechanisms and Clinical Evidence. Clin Dermatol. 2023;41(5):639-648. 

5. Topical Carboxytherapy for Skin Rejuvenation. J Clin Aesthet Dermatol. 2024;17(3):44-51. 

6. Ibrahim Z, et al. Combined Use of Carboxytherapy and PRP in the Periorbital Area. OA Text. 2023. 

7. Makieiev O, et al. The Mechanism of the Pulmoprotective Action of Carboxytherapy (Verigo-Bohr Effect). Eur PMC 2023; PMID: 31513169. 

8. Ferreira M, et al. Carboxytherapy Controls Inflammation and Enhances Wound Healing. Int Wound J. 2024;21(2):e13031. 

9. Minelli L, et al. Carboxytherapy in Non-invasive Aesthetic Medicine and Dermatology. Clinics in Dermatology. 2023;41(6):661-673. 

10. Levitas Academy and Levitas Clinics official pages: levitasacademy.com | levitascliniclondon.com