Leading London and Surrey Carboxytherapy services and training Academy: The Bohr Effect in Carboxytherapy: Oxygen, Regeneration, and Clinical Excellence
Published by Levitas Academy — Training Tomorrow’s Regenerative Clinicians
Clinical applications available at Levitas Clinics London, Esher, and Guildford
Carboxytherapy — the therapeutic use of medical-grade carbon dioxide (CO₂) — has become a cornerstone in regenerative aesthetics. Its effects on microcirculation, collagen stimulation, and tissue oxygenation are rooted in one of physiology’s most elegant principles: the Bohr Effect.
At Levitas Academy, our clinician-led training explores this science in depth, ensuring every practitioner understands the biochemistry behind the treatment. Levitas Clinics, our clinical arm, deliver carboxytherapy within tailored regenerative programs, aligning evidence with real-world outcomes.
The Science: Understanding the Bohr Effect
In 1904, Danish physiologist Christian Bohr described how increased CO₂ levels or decreased pH cause hemoglobin to release oxygen more readily — the Bohr Effect. In simple terms, CO₂ acts as a biological key, unlocking oxygen from hemoglobin and allowing it to diffuse efficiently into tissues.
During carboxytherapy, localised administration of CO₂ increases capillary CO₂ concentration, lowering pH and triggering this rightward shift of the oxygen–hemoglobin dissociation curve. The result: a surge in tissue oxygenation, improved metabolism, and enhanced healing capacity.
Mechanisms of Action in Skin and Tissue Regeneration
- Microvascular Expansion — CO₂ induces vasodilation, improving blood flow and capillary recruitment
- Improved Oxygen Delivery — via the Bohr Effect, correcting tissue hypoxia
- Collagen & Elastin Synthesis — fibroblast activation and controlled hypoxia
- Increased Cellular Metabolism — enhanced healing and regeneration
- Lymphatic Drainage & Detox — supporting inflammatory by-product removal
Clinical Applications
- Periorbital rejuvenation (dark circles, puffiness)
- Cellulite & stretch marks
- Skin laxity & fine lines
- Scarring & post-surgical recovery
- Adjunct in fat grafting & regenerative dermatology
Combination therapies (PRP + CO₂, Exosomes + CO₂) are actively used at Levitas Clinics.
Safety & Tolerability
Carboxytherapy is minimally invasive and safe when performed by trained clinicians. Effects are mild and transient. Contraindications include infection, pregnancy, or uncontrolled vascular disease.
Levitas training ensures anatomical understanding + safety protocols.
Training at Levitas Academy
CPD Accredited Programmes
including Carboxytherapy & The Bohr Effect
Faculty Include:
- Dr. Ash Kapoor — Longevity & Regenerative Physician, Founder
- Dr. Simone Kuter
- Dr. Elizabeth Almas
- Dr. Vijay Prakash
- Dr. Federico Fedel
Certification: CPD hours & suitable for revalidation.
Where Science Meets Practice
Levitas Academy trains medical professionals in mechanisms, safety, and clinical integration. Levitas Clinics apply the same evidence-driven protocols.
Locations covered:
Knightsbridge, Kensington, Chelsea, Park Lane, Belgravia, Mayfair, Marylebone, Westminster, Wimbledon, Epsom, Esher, Guildford, Hazelmere, Farnham, Godalming
Key References
- Jarząbek-Perz S, Wrzosek M. Appl Sci. 2025.
- Bagherani N. J Cosmet Dermatol. 2024.
- Borile G. Springer. 2024.
- Limongi RM. Clin Dermatol. 2023.
- J Clin Aesthet Dermatol. 2024.
- Ibrahim Z. OA Text. 2023.
- Makieiev O. Eur PMC. 2023.
- Ferreira M. Int Wound J. 2024.
- Minelli L. Clin Dermatol. 2023.
- Levitas Academy & Clinic sites
